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. Author manuscript; available in PMC: 2013 Sep 30.
Published in final edited form as: Methods Mol Biol. 2012;849:157–167. doi: 10.1007/978-1-61779-551-0_11

Fig. 2.

Fig. 2

Schematic diagram illustrating analysis of fibril composition or conformation using double immunostaining. (a) Analysis of fibril composition. Amyloid fibrils produced from two homologous proteins (green and red) are treated with a pair of antibodies. Antibodies should be selected using the following rules: one antibody used in a pair (yellow) should recognize either one or both proteins, whereas another antibody (green) should be specific to only one protein, epitopes to both antibodies should be immunoreactive in fibrillar form, (b) Analysis of conformational switching within individual fibils. Amyloid fibrils are treated with a pair of antibodies which are selected using the following rules: the epitope to one antibody used in a pair (green) should be immunoreactive in both amyloid states, whereas the epitope to the second antibody (yellow) should be immunoreactive in only one amyloid state.