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NCI-H460 cell number counted at day 4 of scaffold culture with 3 × 105 (dark grey), 7 × 105 (light grey) and 1.5 × 106 (grey) as initial seeding cell number for 1-2 hours, 4 hours and 24 hours. There is a significant difference between 1-2 hours and 4 hours seeding; *P < 0.05, **P < 0.01.
