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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Mol Cell. 2012 Dec 6;49(2):283–297. doi: 10.1016/j.molcel.2012.10.028

Figure 3. S247 phosphorylation of BAF60c enhances the interaction with USF-1.

Figure 3

(A) Immunoblotting of lysates from 293 cells transfected with Flag-tagged USF-1 and HA-tagged BAF60c wild type (WT), S247A and S247D mutant BAF60c (left) and after IP with USF-1 antibody (right). (B) Lysates of cells transfected with Flag-tagged USF-1 and BAF60c were incubated with either wild type 247 peptide (peptide S247) or peptide containing S247A mutation (peptide S247A) before IP and immunoblotting (left). Lysates from cells transfected with only USF-1 were used (right). (C) GST-USF-1 fusion protein was used for pull-down of BAF60c using glutathione beads (left). GST-USF-1 was incubated with in vitro translated BAF60c in the presence of S247D or S247A peptides. (D) Luciferase activity. Means ± SEM. **P<0.01. (E) ChIP for BAF60c binding by qPCR. Means ± SEM.