Skip to main content
. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Mol Cell. 2012 Dec 6;49(2):283–297. doi: 10.1016/j.molcel.2012.10.028

Figure 5. Post-translational modification of both BAF60c and USF-1 mediates their interaction.

Figure 5

(A) IP of USF-1 with Flag-antibody of 293 cells co-transfected with Flag-tagged USF-1 (WT) or its acetylation mutants (K237R and K237Q) with HA-tagged BAF60 or its phosphorylation mutants (S247A and S247D) and immunoblotting with anti-HA antibody for BAF60c. (B) (top) Input of in vitro translated K237A and K237R mutant USF-1 and wild type and S247A mutant BAF60c. USF-1 and BAF60c were used for Co-IP followed by immunoblotting. (C) ChIP analysis for BAF60c bound to the FAS-Luc promoter in cells transfected with USF-1 or its acetylation mutants along with BAF60c or its mutant by PCR (left) or qPCR (right). (D) Luciferase activity in cells transfected with −444 FAS-Luc, USF-1 and BAF60c or its mutants. Means ± SEM. **p<0.01. (E) Immunoblotting of lysates from insulin-treated HepG2 cells after IP with anti-USF-1 antibody. (F) Immunoblotting of total liver lysates from 6 week-old SCID mice. (G) Immunoblotting of liver nuclear extracts after IP with USF-1 antibody. (H) ChIP for FAS and mGPAT promoters after IP with anti-BAF60c and anti-USF antibodies in livers from SCID mice (left) and quantification by qPCR (right). Means ± SEM.