Table 1.

Hyperfine Interaction Parameters of ³¹P Nuclei in Phosphate Ligands Obtained by Numerical Simulations of the Pulsed ENDOR Spectra Recorded for the Samples of Mn²⁺ with Various Nucleotides, with or without Myosin^a

sample	aP (MHz)	T⊥ (MHz)	ΔaP (MHz)
myosin·Mn·AMPPNP	21.5	−0.9	3.5
Mn·AMPPNP	20.5	−0.9	8
Mn·ATP	22	−0.9	8
myosin·Mn·ADP and Mn·ADP	26	−0.9	14
myosin·Mn·ADP·AlF4	26.5	−0.9	4

^aSee Figure 3. Δa_P is the width of the Gaussian distribution of a_P between the maximum slope points. The true values of a_P and Δa_P are obtained from the respective effective values (that would directly correspond to the ENDOR splittings) by multiplication by 2S = 5, as explained in the text.