Table II.
Western blot results.
| IDO intensity/ GAPDH intensity (% of positive control) | ||||||
|---|---|---|---|---|---|---|
| Median and data range for condition 1, 2, 3, 4, 6 versus 5 (%) |
IDO intensity/GAPDH intensity ratio | |||||
| Condition | Donor | Mean ± SD | Mean ± SD | |||
| 1 | 1 | 119.8 | 61.8 ± 82.1 | 28.3 (3.7–119.8) | 0.246 | 0.13 ± 0.16 |
| 2 | 3.7 | 0.021 | ||||
| 2 | 1 | 34.9 | 21.1 ± 19.4 | 0.071 | 0.05 ± 0.03 | |
| 2 | 7.5 | 0.028 | ||||
| 3 | 1 | 26.4 | 16.2 ± 14.4 | 0.056 | 0.04 ± 0.02 | |
| 2 | 6.0 | 0.031 | ||||
| 4 | 1 | 82.0 | 56.1 ± 36.7 | 0.192 | 0.14 ± 0.07 | |
| 2 | 30.1 | 0.089 | ||||
| 5 | 1 | 2113.3 | 1222.4 ± 1259.91 | 1222.4 (331.5–2113.3) | 4.375 | 2.77 ± 2.271 |
| 2 | 331.5 | 1.170 | ||||
| 6 | 1 | 54.8 | 30.2 ± 34.9 | 0.151 | 0.09 ± 0.09 | |
| 2 | 5.5 | 0.023 | ||||
IDO protein expression in non-polarizing (1, 2, 3, 4, 6) and polarizing (5) DC culture conditions was measured densitometrically. It is shown as a ratio (IDO/GAPDH) in the two right-hand columns and as a percentage of the positive control (IFN-γ-stimulated HeLa cells) in the two middle columns. Mean values ± SD have been calculated for two healthy donors. DC in condition 5 were polarized to DC1 and showed a markedly elevated IDO protein expression compared with non-polarized culture conditions (Mann-Whitney rank sum test, 1P = 0.04).