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. Author manuscript; available in PMC: 2014 Sep 23.
Published in final edited form as: J Mol Biol. 2013 Jul 9;425(18):3461–3475. doi: 10.1016/j.jmb.2013.07.001

Figure 1.

Figure 1

Complexin-I does not bind to the synaptotagmin-1 C2AB fragment. (a) Domain diagram of complexin-I with residue numbers above, and ribbon diagram of the Cpx26-83/SNARE complex37 with SNAP-25 in green, syntaxin-1 in yellow, synaptobrevin in red and Cpx26-83 in orange (accessory helix) and pink (central helix). The N- and C-termini of the region of Cpx26-83 that was observable are labeled with residue numbers, and the N- and C-termini of the SNAREs are indicated by N and C, respectively. (b) ITC analysis of complexin-I binding to the C2AB fragment in 1 mM Ca2+. Complexin-I (150 μM) was titrated into the C2AB fragment (10 μM). (c,d) 1H-15N HSQC spectra of 8 μM 15N-labeled complexin-I in the absence (black) and presence (red) of 10 μM C2AB fragment and 1 mM EDTA (c) or 1 mM Ca2+ (d).