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. Author manuscript; available in PMC: 2014 Sep 23.
Published in final edited form as: J Mol Biol. 2013 Jul 9;425(18):3461–3475. doi: 10.1016/j.jmb.2013.07.001

Figure 5.

Figure 5

Complexin-I increases the solubility of the SNARE complex. SNARE complexes containing non-truncated syntaxin-1 SNARE motif (residues 191–259) were assembled in the absence or presence of complexin-I. For one of the samples, complexin-I was added after assembly. Samples were concentrated 10-fold and diluted 10-fold. Aliquots of the samples before (lanes 1, 3, and 5) or after (lanes 2, 4 and 6) the concetration-dilution procedure were analyzed by SDS-PAGE and commossie blue staining. The position of complexin-I (Cpx) and SNARE complex (SC) in the gels is indicated.