Skip to main content
. Author manuscript; available in PMC: 2013 Sep 30.
Published in final edited form as: J Neurosci Methods. 2008 Oct 21;177(2):294–302. doi: 10.1016/j.jneumeth.2008.10.014

Fig. 5.

Fig. 5

Effect of pulse duration on α1β3γ2S GABAA receptor current responses during repetitive stimulation. (a–d) Representative current responses from 400 μs (panels a and c) and 10 ms (panels b and d) pulses of 1 mM GABA applied at frequencies of 1 Hz (panels a and b) or 20 Hz (panels c and d). Note that there was no appreciable reduction of peak currents at 1 Hz for either pulse length, while there was a gradual reduction of peak current amplitudes at 20 Hz. To facilitate comparison of the rates of peak current reduction during repetitive stimulation, the maximal responses in panels a and c were normalized to those of panels b and d. (e) Representative currents evoked by the first (left) and last (right) pulses in the 20 Hz condition for either 400 μs (black) or 10 ms (grey) pulses. Currents were normalized to the first pulse in the train. (f) Longer GABA pulses are associated with more pronounced loss of peak current amplitude during high frequency repetitive stimulation. *p < 0.05.