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. Author manuscript; available in PMC: 2013 Dec 14.
Published in final edited form as: Immunity. 2012 Dec 6;37(6):986–997. doi: 10.1016/j.immuni.2012.09.014

Figure 1. DNA-IgG-Mediated Production of IFN-α Requires Both FcγR and TLR9.

Figure 1

(A) Bone-marrow-derived pDCs from WT mice were stimulated with increasing concentrations of CG50 plasmid DNA (DNA) in presence of different concentrations of the DNA antibody E11 (DNA Ab). Production of IFN-α was measured by ELISA after 24 hr.

(B) Bone-marrow-derived pDCs from WT and Fcer1g (FcRγ −/−) mice were unstimulated (NS) or stimulated with DNA-IC formed by combining CG50 plasmid DNA and DNA antibody E11 (DNA-IgG). We used 5 μM of ODN1585 (CpG-ODN) as positive control. Secretion of IFN-α was measured by ELISA 24 hr after pDC stimulation. Data is representative of three independent experiments.

(C–F) Fetal liver-derived pDCs from WT, Tlr9−/− (C and E) or Myd88−/− (D and F) mice were stimulated with DNA antibody E11 (IgG), CG50 plasmid DNA (DNA), or DNA-IgG. ODN1585 (CpG-ODN) was used as a positive control. IFN-α and TNF-α in supernatants were measured by ELISA 24 hr after pDC stimulation. Data are presented as mean ± SD of three independent experiments. (*p < 0.05).