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. 2013 Jun 28;6(3):657–662. doi: 10.3892/etm.2013.1187

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Copyright © 2013, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Recombinant plasmid pUCm-T/Eg95. (A) Total RNAs extracted from Echinococcus protoscolex were analyzed on 1.2% MOPS-formaldehyde denaturing gel. (B) Cloning of Eg95 gene from Echinococcus cDNA. Eg95 gene was amplified from Echinococcus cDNA by PCR assay. The PCR products were analyzed by agarose gel electrophoresis. (C) Confirmation of recombinant plasmid pUCm-T/Eg95. Correct construct of pUCm-T/Eg95 plasmid was identified by PCR assay. M, DL2000 DNA marker; lanes 1–3, PCR products of three white colonies.