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. Author manuscript; available in PMC: 2014 Aug 26.
Published in final edited form as: Dev Cell. 2013 Aug 26;26(4):369–380. doi: 10.1016/j.devcel.2013.07.021

Figure 4. NudE migrates with Khc73 in S2 cell.

Figure 4

(A) Fixed interphase S2 cells transfected with myc-Khc73FL and FLAG-NudE preps show that NudE localizes to plus ends of microtubules which is where Khc73 localizes.

(B) Live imaging analysis of interphase S2 cells transfected with GFP-Nude and Khc73FL-mCherry shows colocalization of the two components at the periphery of the cell, presumably to microtubule plus-ends. The two can be seen moving together in S2 cells towards the periphery of the cell.

(C) Live imaging analysis of interphase S2 cells plated on a Con-A coated slide transfected with GFP-NudE and Khc73FL-mCherry. This panel demonstrates an example of the two signals co-migrating towards the plus-end (white arrows).

(D) From the same experiment shown in panel C, an example of the GFP-NudE and Khc73FL-mCherry migrating in opposite directions (white versus yellow arrows). See also Figure S3, Movie S3, and Movie S4.