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. 2013 Sep 30;8(9):e76679. doi: 10.1371/journal.pone.0076679

Figure 2. Progranulin (PGRN) induced activation of the Akt/eNOS/NO pathway in HUVECs.

Figure 2

(A) Cells were incubated with 200 ng/mL of progranulin for 0, 5, 15, 30, or 60 min and analyzed by Western blotting for phosphorylated-eNOS (p-eNOS) level. (B) HUVECs were stimulated with 200 ng/mL of progranulin with or without Akt inhibitor (0.5 or 5 µM) for 15 min, and p-eNOS level was measured by Western blotting. (C) Cells were incubated with 200 ng/mL of progranulin for the indicated times. NO production was analyzed from culture media using the Griess reagent, and OD values were measured at 540nm. Data were obtained from three separate experiments. Error bars represent mean ±SD (n.s.: not significant; *, P< 0.05, **, P< 0.005, t-test)..