Skip to main content
. 2013 Sep 30;8(9):e75689. doi: 10.1371/journal.pone.0075689

Figure 5. Comparison of basal mRNA expression levels and induction by PB of CYP genes in chicken liver.

Figure 5

qRT-PCR was performed with cDNAs derived from chicken liver for following genes: CYP1A4, CYP1A5, CYP1B1, CYP1C1, CYP2C23a, CYP2C23b, CYP2C45, CYP2D49, CYP3A37, and CYP3A80. The transcripts of each gene were calculated using plasmid standards. The values represent the expression levels normalized with GAPDH gene transcripts. (A) basal expression levels in non-treated chicken liver. Data are expressed as an average ± SD (B) fold induction levels by PB are indicated by the ratio of the mRNA expression levels of PB-treated chicken liver and saline-treated chicken liver for each gene. The fold induction level is shown by an average, and the significant induction of genes in PB-treated chicken compared to saline-treated chicken is indicated by an asterisk. N=4 for each group.