Figure 4. IS increase [Ca²⁺]i concentrations in LPS treated J774A.1 macrophages.

Cells were pre-treated with IS for 1 h and then co-treated with LPS and IS (1000–62.5 µM) for 15 minutes. Intracellular calcium concentration was evaluated on J774A.1 cells in Ca²⁺-free medium (panel A). Effect of IS on mitochondrial Ca²⁺ pool was evaluated on J774A.1 cells in Ca2+-free medium in presence of FCCP (0.05 µM) (panel B) after LPS and IS treatment. Results were expressed as mean±s.e.m. of delta (δ) increase of FURA 2 ratio fluorescence (340/380 nm) from three independent experiments. Data were analyzed by analysis of variance test, and multiple comparison were made by Bonferroni's test. # denotes P<0.05 versus control cells (J774A.1 in medium without LPS or IS).