Table 2. Confirmed SNPs in 8325-4 compared to NCTC8325.

Genome positiona	Locus tag (SAOUHSC number)	Annotation	8325 base	8325-4 base	Amino acid change	NRS135b	ISP794c [46]	RN4220c [13]	SH1000c [15]
110019*	00105	Phosphonate ABC transporter, putative substrate-binding protein	C	A	V16F	+		+	+
392716	Intergenic 00389 -00390		G	A		+		+	+
405366	Intergenic 00401 -00402		A	G		+		+
412760*	Intergenic 00411 -00412	Phenol-soluble modulin alpha 3	*	+T	L7fs	+		+
412765*	Intergenic 00411 -00412	Phenol-soluble modulin alpha 3	C	G	A5P	+		+	+
653552	00666	Two-component sensor histidine kinase, GraS	C	G	silent	+		+	+
653801	Intergenic 00666 -00667		C	T		+		+
751285*	00769	Preprotein translocase subunit, SecA	A	T	E449V	+		+	+
827849	00859	Hypothetical protein	A	T	T164S	+		+	+
939304*	00961	Competence transcription factor, ComK	C	T	E53K	+		+	+
954590*	00983	2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylic acid synthase/2-oxoglutarate decarboxylase	C	T	silent				+
1009713*	01041	Pyruvate dehydrogenase E1 component beta subunit, PdhB	G	C	G64A				+
1016979	01048	Spermidine/putrescine ABC transporter, permease protein, putative	G	A	E220K	+		+	+
1020577*	01053	Putative manganese transporter, MntH	G	T	S286STOP	+		+	+
1123048*	01172	Orotate phosphoribosyl transferase, PyrE	G	A	G42S	+		+	+
1358230*	01418	Alpha ketoglutarate dehydrogenase, SucA	C	T	D590N	+		+	+
2106539	02274	ABC transporter, ATP-binding protein, putative	A	T	L602F	+		+	+
2272936*	Intergenic 02448 -02449		A	T
2678563*	02911	Hypothetical protein	T	C	silent	+	+	+
2733480*	02971	Zinc metalloproteinase aureolysin	G	T	Q231K				+

^aAn asterisk indicates that the SNP was included in an extended SNP analysis.

^bThis study

^cThese columns indicate the results of published data