Table 1. Nucleotide sequences of the primers and probes used in this study.

PCR type	Gene name	Primer/Probe name	Sequence（5’–3’)	Amplicon size
Sequence PCR	Acc1	Seq-Acc1-F	TGCATCTGCGCTGTTTGT	184bp
		Seq-Acc1-R	AATGACTGAACACGACGACT
	ALMT1	Seq-ALMT1-F	CCAAGGTGCTTAGGGATC	171bp
		Seq-ALMT1-R	CAAGAAGTGTTGCGGTGA
	Waxy-D1	Seq-Waxy-F	CTATTGGTTCTCCGTGTTTG	263bp
		Seq-Waxy-R	GTCATCTGTCATTTCCTGGTT
	PKABA1	Seq–PKABA1-F	AGGGAGATTTAGCGAGGAT	211bp
		Seq-PKABA1-R	TGTGCCGACAGTTGACTT
Real-time PCR	Acc1	Q-Acc1-F	TGCCCATTGTCGGCCTTA	93bp
		Q-Acc1-R	GGGCAGATGGTTGGAATGC
		Q-Acc1-p	FAM-TGCCTCGACAACACCATCGCTATCC-TAMRA
		Q-Acc1-F2	TGCCCATCGTCGGCCTTA
	ALMT1	Q-ALMT1-F	AATGACTGTGCCGTCTCCAGT	95bp
		Q-ALMT1-R	ACAGAGCCGTGTTCTCTGCA
		Q-ALMT1-p	VIC-CGTGAAAGCAGCGGAAAGCCTCAGA-TAMRA
		Q-ALMT1-F2	ACTGACTGTGCCGTCTCCAGT
		Q-ALMT1-p2	VIC-CGTAAAAGCAGCGGAAAGCCTCAGA-TAMR
	Waxy-D1	Q-Waxy-F	GTCGCGGGAACAGAGGTGT	87bp
		Q-Waxy-R	GGTGTTCCTCCATTGCGAAA
		Q-Waxy-p	FAM-CAAGGCGGCCGAAATAAGTTGCC-TAMRA
	PKABA1	Q-PKABA1-F	CAAGTATGTCATAGAGATTTGAA	87bp
		Q-PKABA1-R	GTAACCGAAGTCACAAATCT
		Q-PKABA1-P	FAM-TCGCACCTCGGCT-MGBNFQ
		Q-PKABA1-p2	FAM-TCGCACCTCGACT-MGBNFQ