Table 1. Nucleotide sequences of the primers and probes used in this study.
PCR type | Gene name | Primer/Probe name | Sequence(5’–3’) | Amplicon size |
---|---|---|---|---|
Sequence PCR | Acc1 | Seq-Acc1-F | TGCATCTGCGCTGTTTGT | 184bp |
Seq-Acc1-R | AATGACTGAACACGACGACT | |||
ALMT1 | Seq-ALMT1-F | CCAAGGTGCTTAGGGATC | 171bp | |
Seq-ALMT1-R | CAAGAAGTGTTGCGGTGA | |||
Waxy-D1 | Seq-Waxy-F | CTATTGGTTCTCCGTGTTTG | 263bp | |
Seq-Waxy-R | GTCATCTGTCATTTCCTGGTT | |||
PKABA1 | Seq–PKABA1-F | AGGGAGATTTAGCGAGGAT | 211bp | |
Seq-PKABA1-R | TGTGCCGACAGTTGACTT | |||
Real-time PCR | Acc1 | Q-Acc1-F | TGCCCATTGTCGGCCTTA | 93bp |
Q-Acc1-R | GGGCAGATGGTTGGAATGC | |||
Q-Acc1-p | FAM-TGCCTCGACAACACCATCGCTATCC-TAMRA | |||
Q-Acc1-F2 | TGCCCATCGTCGGCCTTA | |||
ALMT1 | Q-ALMT1-F | AATGACTGTGCCGTCTCCAGT | 95bp | |
Q-ALMT1-R | ACAGAGCCGTGTTCTCTGCA | |||
Q-ALMT1-p | VIC-CGTGAAAGCAGCGGAAAGCCTCAGA-TAMRA | |||
Q-ALMT1-F2 | ACTGACTGTGCCGTCTCCAGT | |||
Q-ALMT1-p2 | VIC-CGTAAAAGCAGCGGAAAGCCTCAGA-TAMR | |||
Waxy-D1 | Q-Waxy-F | GTCGCGGGAACAGAGGTGT | 87bp | |
Q-Waxy-R | GGTGTTCCTCCATTGCGAAA | |||
Q-Waxy-p | FAM-CAAGGCGGCCGAAATAAGTTGCC-TAMRA | |||
PKABA1 | Q-PKABA1-F | CAAGTATGTCATAGAGATTTGAA | 87bp | |
Q-PKABA1-R | GTAACCGAAGTCACAAATCT | |||
Q-PKABA1-P | FAM-TCGCACCTCGGCT-MGBNFQ | |||
Q-PKABA1-p2 | FAM-TCGCACCTCGACT-MGBNFQ |