Table 3. Ct values of original assays and homogenous assays targeting on the SNPs in five wheat lines with SNPs.
Real-time PCR assay | Primers and probe | Wheat cultivar |
Ct Values
|
||
---|---|---|---|---|---|
50 ng | 5 ng | 1 ng | |||
ALMT1 | Q-ALMT1-F/R and Q-ALMT1-p | Zhongguochun (A>C) | 29.49 | 32.95 | 35.25 |
Zhengmai 9023 (G>A) | 29.34 | 32.89 | 35.93 | ||
Cultivars without SNPs | 27.36-29.21 | 30.77-32.65 | 32.72-35.74 | ||
Q-ALMT1-F2/R and Q-ALMT1-p | Zhongguochun (A>C) | 27.90 | 31.16 | 33.22 | |
Q-ALMT1-F/R and Q-ALMT1-p2 | Zhengmai 9023 (G>A) | 28.14 | 31.40 | 32.52 | |
PKABA1 | Q-PKABA1-F/R and Q-PKABA1-p | Zhoumai 16 (A>G) | 30.29 | 33.39 | 35.40 |
Zimai 12 (A>G) | 30.27 | 33.54 | 35.41 | ||
Cultivars without SNPs | 27.66-29.93 | 30.66-32.66 | 32.49-34.86 | ||
Q-PKABA1-F/R and Q-PKABA1-p2 | Zhoumai 16 (A>G) | 28.60 | 31.70 | 34.17 | |
Zimai 12 (A>G) | 28.91 | 32.17 | 34.64 | ||
Acc1 | Q-Acc1-F/R and Q-Acc1-p | Cannizzo wheat (C>T) | 26.29 | 29.48 | 31.78 |
Cultivars without SNPs | 22.77-24.67 | 25.97-28.49 | 27.83-30.5 | ||
Q-Acc1-F2/R and Q-Acc1-p | Cannizzo wheat (C>T) | 24.39 | 27.78 | 29.19 |