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. 2013 Sep 30;8(9):e76210. doi: 10.1371/journal.pone.0076210

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© 2013 Beazley et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immunostaining for β-catenin (white, red) [nuclei counterstained with DAPI (blue)] and Alcian blue staining for GAG deposition on adjacent sections of aortae from Mgp+/+, untreated Mgp-/-, and quercetin-treated Mgp-/- animals shows that β-catenin protein is detected only in untreated Mgp-/- arterial tissue. Scale = 30 µm. Inset panels show higher magnification of representative nuclei, demonstrating co-localization of β-catenin with DAPI in the Mgp-/- aorta in rounded chondrocyte-like cells surrounded by cartilaginous GAG-rich matrix.