Table 1. Primer sequences for mouse genes analyzed by real-time PCR.
| Gene | Accession # | Forward primer | Reverse primer | |
|---|---|---|---|---|
| β-catenin targets | ||||
| Lrp6 | NM_008514 | ggtgtcaaagaagcctctgc | gctcgaggactgtcaaggtc | |
| Tcf4 | NM_013685 | cgaaaagttcctccgggtttg | cgtagccgggctgattcat | |
| Numb | NM_010949 | cttcccagttaagtacctcggc | cccgtttttccaaagaagcct | |
| CyclinD1 (cycD1) | NM_007631 | gcgtaccctgacaccaatctc | ctcctcttcgcacttctgctc | |
| Chondrogenic markers | ||||
| Sox9 | NM_011448 | gttgtaacaccagcagcgtcaag | tgacatactccactttggccacct | |
| Type II collagen (Col II) | NM_031163 | caccgaaagtttaagcacaccca | aaataaccctgcccacactcttg | |
| Aggrecan (Agg) | NM_007424 | atgccacaagtcacagaaaccacg | aaggcagtcacagcattgttgagc | |
| Indian hedgehog (Ihh) | NM_010544 | cgtgaccgaaataagtatggact | gctgctggttctgtatgattg | |
| MMP13 | NM_008607 | tggagtgcctgatgtgggtgaata | tggtgtcacatcagaccagacctt | |
| TG2 | NM_009373 | aggtgtccctgaagaacccacttt | ttccacagacttctgctccttggt | |
| Housekeeping genes | ||||
| Ribosomal protein L19 (Rpl19) | NM_009078 | aagaggaagggtactgccaatgct | tgaccttcaggtacaggctgtgat | |
| beta-actin | NM_007393 | taatttctgaatggcccaggtct | ctggctgcctcaacacctcaa | |