Figure 3. Effects of mannosylated lipoarabinomannan (Man-LAM) on acidification of phagosomes containing Mycobacterium avium subsp. avium .

Bovine macrophages (1×10⁶/ml) were incubated with or without Man-LAM (5 µg/ml) for 2 h and then were incubated with Mycobacterium avium subsp. avium for 18 h. The ratio of red (i.e., acidified phagosome) to green fluorescence (i.e., non-acidified phagosome) of at least 100 phagosomes was determined by use of confocal microscopy. Asterisk indicates statistically significant difference between the treated and untreated group using the Student's t-test. P<0.05 was considered statistically significant.