Figure 1.

Map of vector pIT2 harboring a single-chain variable fragment (scFv)-encoding insert. RBS, ribosome binding site; PelB, signal sequence; VH-linker-VL, scFv; His-tag, immunopurification tag. A TAG amber stop codon was present at the junction of the scFv gene and gIII. The presence of an amber stop codon allows the production of scFv molecules as soluble antibody molecules instead of scFv-pIII fusion proteins. The size of the empty vector pIT2 was 4.2 kb, while the size of the scFv insert was ∼750 bp.