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. 2013 May 30;6(2):552–556. doi: 10.3892/etm.2013.1132

Figure 5.

Figure 5.

Expression and purification of the diabody (10% SDS-PAGE). Lane 1, total protein of bacterial pIT2-A1G9 prior to induction; lane 2, total protein of bacterial pIT2-A1G9 induced with IPTG; lane 3, periplasmic lysates of bacterial pIT2-A1G9 induced with IPTG; lane 4, cell lysate precipitate of bacterial pIT2-A1G9 induced with IPTG; lane 5, the Ni-NTA column flow-through; lane 6, the eluate washed at a concentration of 30 mM imidazole; lane 7, the eluate washed at a concentration of 500 mM imidazole; lane M, protein marker. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; IPTG, isopropylthio-β-galactoside.