Figure 5. Progenitor and proliferation marker expression of RPC cultures during differentiation.

After seven days in the differentiation medium, the cells were fixed and immunostained with antibodies against nestin (A-D) and ki-67 (E-H). The proportion of nestin- and ki-67-positive cells showed no significant difference between different groups and was less than 15% (I). The quantification of immunoreactive cells was performed as described in Figure 3-I. *P<0.05. Scale bars: 50 µm.