Table 1. Primers used for quantitative RT-PCR.
Genes | Accession no. | Forward (5’–3’) | Reverse (5’–3’) | Annealing temperature (°C) | Product size (base pairs) |
---|---|---|---|---|---|
Pax6 | NM_013627 | agtgaatgggcggagttatg | acttggacgggaactgacac | 60 | 132 |
Mash1 | NM_008553 | tctcctgggaatggactttg | ggttggctgtctggtttgtt | 60 | 142 |
Nestin | NM_016701 | aactggcacctcaagatgt | tcaagggtattaggcaagggg | 60 | 235 |
Vimentin | NM_011701 | tggttgacacccactcaaaa | gcttttggggtgtcagttgt | 60 | 269 |
Ki-67 | X82786 | cagtactcggaatgcagcaa | cagtcttcaggggctctgtc | 60 | 170 |
Betacellulin | NM_007568 | cacaggtaccacccctagaca | cctttctcacagatgcaggag | 60 | 135 |
TLX | NM_152229.2 | cgattagacgccactgaa | ggtatctggtatgaatgtagc | 60 | 166 |
Map2 | NM_001039934 | agaaaatggaagaaggaatgactg | acatggatcatctggtaccttttt | 60 | 112 |
β3-tubulin | NM_023279 | cgagacctactgcatcgaca | cattgagctgaccagggaat | 60 | 152 |
PKC-α | NM_011101 | cccattccagaaggagatga | ttcctgtcagcaagcatcac | 60 | 212 |
AP2α | NM_001122948.1 | gccgtccacctagccaggga | gattgggccgcgagttcccc | 60 | 208 |
Brn3a | NM_011143.4 | cgctctcgcacaacaacatga | ttcttctcgccgccgttga | 60 | 121 |
GFAP | NM_010277 | agaaaaccgcatcaccattc | tcacatcaccacgtccttgt | 60 | 184 |
Rhodopsin | NM_145383 | tcaccaccaccctctacaca | tgatccaggtgaagaccaca | 60 | 216 |
β-actin | NM_007393 | agccatgtacgtagccatcc | ctctcagctgtggtggtgaa | 60 | 152 |