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. 2013 Sep 30;8(9):e76157. doi: 10.1371/journal.pone.0076157

Table 1. Primers used for quantitative RT-PCR.

Genes Accession no. Forward (5’–3’) Reverse (5’–3’) Annealing temperature (°C) Product size (base pairs)
Pax6 NM_013627 agtgaatgggcggagttatg acttggacgggaactgacac 60 132
Mash1 NM_008553 tctcctgggaatggactttg ggttggctgtctggtttgtt 60 142
Nestin NM_016701 aactggcacctcaagatgt tcaagggtattaggcaagggg 60 235
Vimentin NM_011701 tggttgacacccactcaaaa gcttttggggtgtcagttgt 60 269
Ki-67 X82786 cagtactcggaatgcagcaa cagtcttcaggggctctgtc 60 170
Betacellulin NM_007568 cacaggtaccacccctagaca cctttctcacagatgcaggag 60 135
TLX NM_152229.2 cgattagacgccactgaa ggtatctggtatgaatgtagc 60 166
Map2 NM_001039934 agaaaatggaagaaggaatgactg acatggatcatctggtaccttttt 60 112
β3-tubulin NM_023279 cgagacctactgcatcgaca cattgagctgaccagggaat 60 152
PKC-α NM_011101 cccattccagaaggagatga ttcctgtcagcaagcatcac 60 212
AP2α NM_001122948.1 gccgtccacctagccaggga gattgggccgcgagttcccc 60 208
Brn3a NM_011143.4 cgctctcgcacaacaacatga ttcttctcgccgccgttga 60 121
GFAP NM_010277 agaaaaccgcatcaccattc tcacatcaccacgtccttgt 60 184
Rhodopsin NM_145383 tcaccaccaccctctacaca tgatccaggtgaagaccaca 60 216
β-actin NM_007393 agccatgtacgtagccatcc ctctcagctgtggtggtgaa 60 152