Table 2. Primary antibodies used for immunocytochemistry.
Antibodies | Type | Specificity in retina | Source | Dilution |
---|---|---|---|---|
Nestin | Mouse monoclonal | Progenitors reactive glia | BD | 1:200 |
Ki-67 | Mouse monoclonal | Proliferating cells | BD | 1:200 |
Map2 | Rabbit monoclonal, | Neurons | Epitomics | 1:200 |
β3-tubulin | Mouse monoclonal | Neurons | Chemicon | 1:100 |
AP2α | Mouse monoclonal | Amacrine cells | DSHB | 1:600 |
Brn3a | Rabbit polyclonal | Ganglion cells | Millipore | 1:500 |
PKC-α | Mouse monoclonal | Bipolar cells | BD | 1:200 |
GFAP | Mouse monoclonal | Glia | Chemicon | 1:200 |
Rhodopsin | Mouse monoclonal | Photoreceptors (rods) | Chemicon | 1:100 |