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. 2013 Sep 30;8(9):e76186. doi: 10.1371/journal.pone.0076186

Figure 9. A short GARP 3’ UTR region controls GARP levels and production of TGF-β1 in T cells.

Figure 9

Clone Th A2 was transduced with lentiviruses containing the GARP coding sequence (GARP ORF) alone, or followed by a full length (FL) or a truncated form (1.7 kb) of the GARP 3’ UTR. Transduced cells were restimulated for 24 hours with anti-CD3/CD28 antibodies in serum-free medium.

A to C. Cell lysates were analysed by WB after SDS-PAGE in reducing conditions with antibodies against GARP, β-ACTIN (A) and a C-terminal epitope of TGF-β1 (C), and by RT-qPCR for GARP and housekeeping gene EF-1 (B). Values in B are means of duplicates + SD. D. Concentration of latent TGF-β1 in the supernatants was measured by ELISA as indicated in Figure 2. Values are means of duplicates + SD, and are representative of 3 independent experiments. Similar results were obtained with unstimulated cells.