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. 2013 Sep 30;8(9):e75669. doi: 10.1371/journal.pone.0075669

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© 2013 Friend et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Proteins pulled down from rat brain lysate using either unlabelled or dTelo4 oligonucleotide-labelled magnetic beads were resolved by SDS-PAGE and either stained with Coomassie Blue G250, or transferred to an Immobilon-Fl membrane for Western blotting. HnRNPs A1 and A2/B1 were identified by the application of anti-hnRNP A1 (Sigma, clone 4B10) followed by secondary anti-mouse IgG IRD800 or anti-rabbit A2/B1 [12] followed by secondary anti-rabbit IgG Alexa-680 fluorophore.