Table 1. Comparison between BNP2 and Rituximab effects.

	CD20	BNP2	RITUXIMAB
	(MFI)	(% killing)	(% killing)
BJAB	316,8	92,0	18,9
BJAB-High	602,9	93,2	20,6
BJAB-low	103,1	83,1	0,6
Pz1	73,6	77,4	0,0
Pz2	22,2	81,7	8,9
Pz3	25,3	55,1	9,9
Pz4	41,4	44,9	18,0
Pz5	64,5	47,9	21,8
Median	41,4	55,1	9,9

BJAB cells were sorted to obtain High-CD20 and Low-CD20 cells. Mononuclear cells were purified from untreated CLL patients. Cells were analyzed for CD20 expression (MFI-mean fluorescence intensity) and then incubated with BNP2 for 48 hours to induce apoptosis. Cells were also incubated with Rituximab + NHS as a source of Complement for 1 h to induce CDC. Residual viable cells were measure using MTT assay.