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. 2013 Sep 30;8(9):e75691. doi: 10.1371/journal.pone.0075691

Figure 8. Sc-KU-4 targeted host pattern recognition proteins.

Figure 8

Insect plasma proteins were captured by recombinant Sc-KU-4 after performing a pull-down protein, eluted and analyzed by 2DE. A: Two proteins were detected in the eluate obtained from the Sc-KU-4-coupled beads with an acidic gradient. B: Seven proteins were detected in the eluate obtained with a salt gradient. Isofocalization were performed on 3-10 NL IPG strips, and the second dimension was run on a 12% SDS-PAGE gel. Proteins were stained with colloidal Coomassie blue. MM is shown in kDa. C: Sc-KU-4 targeted proteins identified by MS/MS.

a: Protein score probability limit (P < 0.05) of 85; b: Only peptides with confidence intervals above 95% were considered; c: Sequence coverage of identified peptides; d: Number of peptides identified by MS/MS.