Figure 5. IFN-γ and GzmA expression after ACM stimulation.

Human peripheral blood mononuclear cells (PBMCs) isolated from six leukocyte filters in each case were cultured for 24 hours with R10-medium conditioned with 1% of SGBS ACM harvested on day 7 (d7) or day 11 (d11) after induction of adipogenesis. PBMCs incubated for 24 hours with medium only served as controls. A detailed description of the incubation procedure can be found in the materials and methods section. A) IFN-γ-expressing human blood CD56^dim and CD56^bright NK cells as percentage of human blood CD56^dim and CD56^bright NK cells treated with 1% of the ACM harvested on day 7 after induction of adipogenic differentiation; B) GzmA-expressing human blood CD56^dim NK cells as percentage of human blood CD56^dim NK cells treated with 1% of the ACM harvested on day 7 or 11 after induction of adipogenic differentiation; C) GzmA-expressing human blood CD56^bright NK cells as percentage of human blood CD56^bright NK cells treated with 1% of the ACM harvested on day 7 or 11 after induction of adipogenic differentiation; Statistically significant differences between the stimulation with SGBS ACM and the medium control are depicted with an asterisk (*). Statistically significant differences between the stimulation with SGBS ACM harvested on day 7 after induction of adipocyte differentiation and the same dosage of SGBS ACM harvested on day 11 after induction of adipogenic differentiation are depicted with a rhomb (#). Statistically significant differences between the numbers of IFN-γ expressing CD56^dim and CD56^bright NK cells are depicted with a paragraph sign (§). All data represent means+SEM.