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. 2013 Sep 30;8(9):e75703. doi: 10.1371/journal.pone.0075703

Figure 6. Ob-R, TRAIL and IFN-γ expression after stimulation with either murine or human ACM.

Figure 6

PBMCs were cultured for 24 hours with R10-medium conditioned with 1% of either murine 3T3-L1 or human SGBS ACM harvested on day 7 after induction of adipocyte differentiation. PBMCs incubated for 24 hours with medium only served as controls. A detailed description of the incubation procedure can be found in the materials and methods section. A) Ob-R-expressing human blood NK cells as percentage of human blood NK cells; B) TRAIL-expressing human blood NK cells as percentage of human blood NK cells; C) IFN-γ-expressing human blood NK cells as percentage of human blood NK cells. Statistically significant differences between the stimulation with 3T3-L1 or SGBS ACM and the medium control are depicted with an asterisk (*). Statistically significant differences between the stimulation with SGBS ACM and 3T3-L1 ACM are depicted with a rhomb (#). All data represent means+SEM.