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. 2013 Sep 30;8(9):e75703. doi: 10.1371/journal.pone.0075703

Table 1. Lymphocyte numbers after 24 hours of stimulation with ACM (adipocte-conditioned medium).

Medium SEM 0.1% ACM SEM 1% ACM SEM 10% ACM SEM
NK cells 13.1 2.2 14.1 3.6 14.3 3.7 14.1 3.6
CD56dim NK cells 93.2 1.0 93.3 1.4 92.9 1.4 92.7 1.8
CD56bright NK cells 6.8 1.0 6.7 1.5 7.1 1.4 7.3 1.8
T cells 59.9 3.0 60.8 2.9 60.9 3.1 61.2 2.8
NKT cells 3.4 1.0 2.4 0.7 2.3 0.7 2.4 0.7

Human peripheral blood mononuclear cells (PBMCs) were isolated from six leukocyte filters and cultured for 24 hours with R10-medium conditioned with 0.1%, 1% or 10% of SGBS ACM. PBMCs incubated for 24 hours with medium only served as controls. A detailed description of the incubation procedure can be found in the materials and methods section. NK cells, T cells and NKT cells are shown as percent of lymphocytes. CD56dim and CD56bright NK cells are shown as percent of all NK cells.