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Identification of α-N-methylation of CENP-B. (A) ESI-MS of the N-terminal peptide GPKRRQLTRE from the Glu-C digestion of C-terminally FLAG-tagged human CENP-B. (B) and (C) MS/MS of unmodified (B) and tri-methylated N-terminal peptide (C) of CENP-B.
