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. 2013 Jul 14;4(8):1185–1198. doi: 10.18632/oncotarget.1162

Figure 3. TRAIL response time course in RKO colorectal cancer and CO841 non-transformed colon epithelial cells.

Figure 3

Panel A: images of RKO cells at time 0 or treated with TRAIL 200 ng/ml for 2, 4, 8, 24 h or 24 h plus additional 4 h with fresh 200 ng/ml TRAIL were taken using an inverted light microscope (Leica), 20X magnification. Some rounded and small apoptotic cells were detected at 2, 4 and 8h. Cleavage of PARP was assessed by immunoblot at time 0 (C), 2, 4, 8, 12 and 24 plus additional 4 h 200 ng/ml TRAIL treatment (panel B, left). Panel B, right, shows the effects of the same treatment in TRAIL-pretreated RKO cancer cells after 4 days of recovery. Panel C shows results for a similar time course in non-transformed CO841 colon epithelial cells.