Skip to main content
. 2013 Jul 14;4(8):1199–1211. doi: 10.18632/oncotarget.1122

Figure 4. Annexin A2 promotes OV-90 cell motility and invasion in vitro and in vivo.

Figure 4

(A) Cell motility and invasion of OV-90 cells treated with mouse IgG antibody or annexin A2 neutralizing antibody in vitro. Data are expressed as a percentage of control mouse IgG, mean ± SEM of quadruplicates from 3 independent experiments (n=12). *, significantly different from control (P < 0.05, Student t-test). (B) Chick chorioallantoic membrane (CAM) implanted with matrigel and OV-90 cancer cells (CMI) at day 14 of chick embryo development. The CAM layers ectoderm (ECT), mesoderm (MES) and endoderm (END) are evident. Haematoxylin and eosin and pan-cytokeratin immunohistochemistry of the CAM implanted with OV-90 cells in the presence of mouse IgG antibody (C,D) and annexin A2 neutralizing antibody (E,F). (G) Quantitative analysis of OV-90 cells invasion into the CAM. Data represents mean ± SEM from 72-80 images (n=12 chick embryos per treatment group from 2 independent experiments). *, significantly different from control (P < 0.05, Mann-Whitney U test). Magnification bar = 100 μm for all images.