Figure 2. The Mcl1L mRNA variant Mcl1L_JAM exerts anti-proliferative effect.

SH-EP/Ctr, SH-EP/Mcl1L and SH-EP/Mcl1L_JAM cells were treated for four hours with 200 nM bortezomib (bort). Cell lysates were subjected to immune blot to verify ectopic expression of Mcl1L and Mcl1L_JAM. α-Tubulin served as a loading control (a). Colony formation was analysed by crystal violet staining of 2×10³ SH-EP/Ctr, SH-EP/Mcl1L and SH-EP/Mcl1L_JAM cells after 7 days cultivation (b, upper and lower panel). Bright field analyses of single colonies were performed in an Axiovert200M microscope (b, middle panel). Shown is the mean of four independent experiments. Statistical analysis was performed using students t-test (* P < 0.05).