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. 2013 Jul 15;4(8):1241–1252. doi: 10.18632/oncotarget.1147

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Copyright: © 2013 Hagenbuchner et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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SH-EP/Ctr, SH-EP/Mcl1L and SH-EP/ Mcl1L_JAM cell lysates were analysed for phosphorylated Mcl1 (Ser159/Thr163) and Mcl1 after treatment with bortezomib for four hours (a). α-Tubulin served as loading control. Protein stability was analysed by treating SH-EP/Ctr, SH-EP/Mcl1L or SH-EP/Mcl1L_JAM cells with 10 μg/ml CHX for the times indicated (b). α-Tubulin served as loading control. SH-EP/Ctr, SH-EP/Mcl1L and SH-EP/Mcl1L_JAM cells were treated for four hours with 200 nM bortezomib. Cell lysates were analysed for the expression of Noxa and Bim. GAPDH served as loading control (c).