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. 2013 Oct 1;7:153. doi: 10.3389/fncir.2013.00153

Figure 2.

Figure 2

Defining pre- and post-synaptic spots. (A,D) show low and high power magnification of post-synaptic PSD-95 (purple) fluorescence in the entire confocal z-stack from Figure 1. (B,E) show the post-synaptic PSD-95 (purple) fluorescence filtered to inside the neuronal surface using the “mask all” tool in Imaris. (G,J) show low and high power magnification of pre-synaptic VGLUT2 (green) fluorescence in the entire confocal z-stack from Figure 1. (H,K) show the pre-synaptic VGLUT2 (green) fluorescence filtered to outside the neuron surface using the “mask all” tool in Imaris. (C,F,I,L) show low and high-powered magnifications of post-synaptic PSD-95 (purple) and pre-synaptic VGLUT2 (green) identified using the “create spots” algorithm in Imaris. Scale bars: (A,B,C,G,H,I) 5 μm and (D,E,F,J,K,L) 2.5 μm.