Table 1.
Location | VGAT spots (n = 6) | GABA-Aα1 spots (n = 6) | VGLUT2 spots (n = 4) | PSD95 spots (n = 4) |
---|---|---|---|---|
Soma + proximal dendrites | 212.1 ± 52.8 | 159.7 ± 38.2 | 39.3 ± 7.7 | 38.8 ± 9.1 |
Distal dendrites | 18.8 ± 3.5 | 20.7 ± 4.3 | 41.8 ± 10.9 | 40.8 ± 7.7 |
Spots are quantified using the method described above, with VGAT and VGLUT2 pre-synaptic spots being within 1 μm of a GABA-A α1 receptor subunit or PSD-95 spot respectively. Data presented as mean ± SEM. Below, we quantify the number of spots of each type found in our sample at representative parts of the motor neuron; there is a higher proportion of inhibitory synapses found on the soma and proximal dendrites (PDs), while there is a higher proportion of excitatory synapses found on the distal dendrites.