Table 2.

Specificity of immunocytochemistry.

Antibodies	Spots	Spots within 1 μm (co-label)	% co-label
PSD-95 within cell n = 4	52.7 ± 13.9	7.5 ± 2.9 (GABA α1 co-label)	14.2
GABA-A α1 within cell n = 4	209.3 ± 55.4	12 ± 2.7 (PSD-95 co-label)	5.7
VGLUT2 within 1 μm of cell n = 3	466 ± 107.7	49 ± 26 (VGAT co-label)	10.5
VGAT within 1 μm of cell n = 3	473.4 ± 139	62.3 ± 26.1 (VGLUT2 co-label)	13.2

Table show spots of excitatory (PSD-95) and inhibitory (GABA-A α1) post-synaptic specializations within the same Neurobiotin-filled neuron somas and the number of spots of each synapse type within 1 μm of each other. Also shown are spots of excitatory (VGLUT2) and inhibitory (VGAT) pre-synaptic specializations within 1 μm of Neurobiotin-filled neuron somas and the number of spots of each synapse type within 1 μm of each other. Data presented as mean ± SEM.