The identification of the putative DMR regions of three bovine imprinted genes using density plotting of CpG sites. (A) The defined IGF2 DMR is located in intron 9/exon 10. The defined XIST and H19 DMRs are located in the promoter/exon 1 region. (Open box) gene exon region; (solid bar) putative DMR. The vertical line indicates the CpG sites. (B) The normal methylation patterns of the three imprinted genes in several tissues of three wild-type (WT) bovines. The methylation status of IGF2, XIST, and H19 was assayed by COBRA. He, heart; Li, liver; Sp, spleen; Lu, lung; Ki, kidney; Mu, muscle.