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. 2013 Oct 1;4:271. doi: 10.3389/fphys.2013.00271

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Copyright © 2013 Csaderova, Debreova, Radvak, Stano, Vrestiakova, Kopacek Pastorekova and Svastova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The impact of CA IX expression on adhesion and spreading of stably transfected MDCK cells. (A) MDCK cells were seeded on collagen-coated substrate and fixed at indicated time points. Expression of full length CA IX enhanced spreading area when compared with control neo cells. MDCK cells expressing Δ PG mutant decreased spreading area against cells expressing intact CA IX. Cell area was normalized to compensate for different cell sizes of used transfectant and control cell lines. (B) Addition of M75 antibody targeting the PG domain of CA IX led to the reduction of spreading area of adhering MDCK CA IX cells on glass coverslips. Both graphs show the mean ± standard deviation values for at least 200 cells, significance of differences among samples was tested by t-test (^**p < 0.01).(C) Presence of M75 antibody inhibits the rate of cell spreading.