FIG. 2.

Immunofluorescence characterization of pNPCs. (A) Phase-contrast photograph of cells isolated from porcine fetal brain. Neurospheres are seen. Scale bars, 100 μm. (B) Neurospheres labeled with antibodies as labeled. (C and D) Differentiated pNPCs were stained with TUJ1 (red), GFAP (green), and DAPI (blue). Cells were TUJ1+/GFAP−, TUJ1−/GFAP+, or TUJ1+/GFAP+. Arrows shows TUJ1+/GFAP+ cells. (E) Differentiated pNPCs were stained with A2B5 (red) and DAPI (blue). Color images available online at www.liebertpub.com/cell