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. 2013 Sep 30;202(7):1041–1055. doi: 10.1083/jcb.201302139

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© 2013 Kotani et al.

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Figure 5. — Mouse and zebrafish Pum1 proteins associate with cyclin B1 mRNAs. (A) Schematic diagrams of mouse and zebrafish cyclin B1 3′UTRs. Green rectangles indicate putative PBEs, and red rectangles indicate the poly(A) signal. (B, top) Immunoblotting of mouse ovary extracts before IP (Initial) and IP with goat IgG (IgG) or anti-Pum1 goat antibody (α-Pum1). (bottom) RT-PCR amplification for cyclin B1, mos, β-actin, and α-tubulin transcripts. (C, top) Immunoblotting of zebrafish ovary extracts before IP (Initial) and IP with rabbit IgG (IgG) or anti-Pum1 rabbit antibody (α-Pum1). (bottom) RT-PCR amplification for cyclin B1, mos, β-actin, and α-tubulin transcripts. Similar results were obtained from three independent experiments.