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. 2013 Sep 30;202(7):1107–1122. doi: 10.1083/jcb.201307084

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© 2013 Petit et al.

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Figure 1. — FLCN controls TFEB-dependent regulation of lysosomes. (A) Spinning disk confocal imaging of TFEB-GFP localization ± FLCN knockdown. (B) Quantification of the effect of FLCN knockdown on the nuclear/cytoplasmic ratio of TFEB-GFP (n = 3 experiments, mean ± SEM; *, P < 0.05 by ANOVA with Bonferroni post-test). (C) Representative Western blots of anti-GFP immunoprecipitations from a stable TFEB-GFP HeLa cell line ± FLCN knockdown (n = 3). (D) Lysotracker labeling of HeLa cells transfected with the indicated siRNAs or after treatment with torin 1 (250 nM, 24 h). (E) Quantification of Lysotracker staining (n = 3 experiments, ANOVA with Bonferroni post-test, mean ± SEM; **, P < 0.01; ***, P < 0.001). Bars: (A and D) 10 µm. All images acquired by spinning disk confocal microscopy.