Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 30;202(7):1107–1122. doi: 10.1083/jcb.201307084

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Petit et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — FLCN and FNIP1 colocalize on lysosomes. (A) Localization of FLCN-tdTomato (red, diffuse) and LAMP1-mGFP (green, lysosomes). (B) FLCN-interacting protein 1 (FNIP1)-GFP (green) localizes to Alexa 647 dextran-positive (magenta) lysosomes. (C) Cells cotransfected with FLCN-tdTomato (red) and FNIP1-GFP (green) whose lysosomes were loaded with Alexa 647 dextran (magenta). (D) FLCN-tdTomato and FNIP1-GFP colocalization clearly occurs on the surface of the enlarged endosomes/lysosomes that form in vacuolin-treated (5 µM, 1 h) cells. All images were obtained by spinning disk confocal microscopy of live HeLa cells. Bars: (A–D) 10 µm; (insets) 5 µm.