Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 30;202(7):1107–1122. doi: 10.1083/jcb.201307084

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Petit et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Regulation of the lysosomal recruitment of FLCN by amino acid availability and FNIP1. (A) Western blot analysis of the 3xHA-tagged endogenous FLCN and phosphorylated S6 protein ± FLCN knockdown. (B) Representative immunofluorescence experiment showing the effect of FLCN knockdown on the levels of endogenous 3xHA-FLCN. (C) Representative images showing the effect of starvation (2 h serum and amino acid–free RPMI) and amino acid re-feeding (1× MEM amino acid solution, 15 min) on 3xHA-tagged endogenous FLCN and LAMP1 in control conditions or (D) after FNIP1 knockdown. All images obtained by line scanning confocal microscopy. Bars: (B–D) 10 µm; (inset) 5 µm. All experiments were performed in HeLa cells.