Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 30;202(7):1057–1073. doi: 10.1083/jcb.201210123

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Luo et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Localization and dynamics of actin nodes and myosin II. (A) The dynamic reorganization of F-actin and myosin II was visualized using Lifeact-Ruby and EGFP-tagged MRLC (EGFP-MRLC). MEFs were plated on fibronectin-patterned glass coverslips and then treated with 200 nM LatA. The cell area was marked as the yellow contour. Regions marked in white boxes are magnified in B and show the dynamic reorganization of actin and MRLC. Actin nodes and MRLC first exhibited an alternating pattern (0–20 s, yellow arrows), but the degree of colocalization increases with time. (C) The 3D kymograph of time-lapsed images in B. As multiple actin node fusion and fission events occurred, zigzag patterns appeared in the 3D kymograph. Bars: (A) 10 µm; (B) 2 µm; (C) 5 µm.