FIG. 2.

SD-hESCs efficiently differentiate into primitive endoderm. Undifferentiated hESCs and SD-hESCs and day-15 EBs derived from each of these respective types of hESCs were analyzed for expression of OCT4 (green; a pluripotent cell marker), GATA6 (red; a primitive endoderm marker (red) (A), and TTR (red; a visceral endoderm marker) (B). Nuclei were counterstained with DAPI (blue). Scale bar represents 50 μm. EBs, embryoid bodies; TTR, transthyretin. Color images available online at www.liebertpub.com/scd